ZENIT•AMiDot slides can be run either manually or on automated IFA slide processors
The principal steps of the method are:
Serum sample dilution (1/80) and incubation with autoimmune antigen array fixed on glass slides for 30 min in a moisture chamber at RT
Incubation with flurophore conjugated goat anti-human IgG/IgA for 30 min at RT
Assessment: automated analysis of slides
Signal intensity is directly proportional to the amount of antibody present in the sample. Results are expressed in U/ml or IU/ml.
The information contained in this section is intended for the exclusive use of healthcare professionals.
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